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Home All anti-TP53 antibodies
Anti-TP53 Antibody E47
TrueMAB™ Antibodies - Made against Authentic Protein Antigens
* Buy one primary antibody, get 50% off a GAPDH loading control antibody.
Also for TP53 (NM_000546)
OriGene Data
| A synthetic peptide corresponding to C-terminal residues human p53 was used as immunogen. The antibody should recognize human wild-type and mutant p53. |
| E47 |
Rabbit IgG |
| Human |
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| WB, FC |
WB: 1:1,000; ICC: 1:100; FC: 1:50; IP: 1:50 |
| 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
| (Protein A or G Sepharose) |
Reference Data
| Homo sapiens tumor protein p53 (TP53), transcript variant 1 |
| BCC7; LFS1; P53; TRP53 |
| NP_000537 |
| This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq]. |
Apoptosis Cell cycle Delta-Notch Signaling Pathway MAPK signaling pathway Senescence and Autophagy TGF Beta Signaling Pathway Wnt Signaling Pathway |
* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
WB Image
Western blot analysis on A431 cell lysate using anti-p53 RabMAb (TA303724), 1:1,000 dilution.
FC Image
Flow cytometric analysis of permeabilized A431 cells using anti-p53 RabMAb (red) (TA303724) or a rabbit IgG (negative)(green).
Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:
- HRP
- Biotin
- PE
- FITC
- DyLight® 488
- DyLight® 550
- DyLight® 594
- DyLight® 633
* DyLight is a trademark of Thermo-Fisher Scientific
Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.
Contact us with your conjugation/bulk request: antibody@origene.com or 888-267-4436 (Tech Support)
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