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Anti-TOP2A PHOSPHO Antibody EP1054Y
Also for TOP2A (NM_001067)
|A synthetic phospho-peptide corresponding to residues surrounding Thr 1343 of human Topo II alpha was used as immunogen. The antibody only detects Topo II alpha phosphorylated on Threonine 1343.|
||Tissue culture supernatant
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||IHC-P: Use at an assay dependent dilution; WB: 1:1000; ICC: 1:50 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens topoisomerase (DNA) II alpha 170kDa (TOP2A)|
Entrez Gene 7153 Human
|This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq]. |
|Druggable Genome |
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Western blot - Topoisomerase II alpha (phospho T1343) antibody [EP1054Y]; All lanes : Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] at 1/1000 dilution.Lane 1 : HeLa cell lysate treated with AP.Lane 2 : HeLa cell lysate untreated.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 174 kDa.Observed band size : 174 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Topoisomerase II alpha (phospho T1343) antibody [EP1054Y]; TA303722 at 1/100 dilution staining Topoisomerase II alpha in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.