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Anti-TK1 Antibody EPR3193
|A synthetic peptide corresponding to residues in human Thymidine Kinase 1 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||ICC/IF: 1:100; WB: 1:5000 - 1:50000; IP: 1:10; IHC-P: 1:100 - 1:250; FC: 1:70
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens thymidine kinase 1, soluble (TK1)|
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Western blot - Thymidine Kinase 1 antibody [EPR3193]; Anti-Thymidine Kinase 1 [EPR3193] antibody at 1/50000 dilution + Molt-4 cell lysate at 10 µg.Secondary.HRP labelled goat anti-rabbit at 1/1000 dilution.Predicted band size : 25 kDa.Observed band size : 25 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Thymidine Kinase 1 antibody [EPR3193]; Immunohistochemical analysis of paraffin-embedded human tonsils using 1/100 ab76495
Flow Cytometry-Anti-Thymidine Kinase 1 antibody [EPR3193](ab76495); Overlay histogram showing HeLa cells stained with ab76495 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.