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Home Antibody All anti-TH antibodies

Anti-TH Antibody EP1533Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303716
  • Rabbit monoclonal antibody against Tyrosine 3-Hydroxylase (EP1533Y )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC424777) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for TH (NM_000360)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues surrounding serine 70 human Tyrosine 3-Hydroxylase was used as an immunogen.
Clone NameEP1533Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:500; ICC: 1:100 - 1:250; FC: 1:20; IHC-P: 1:200
Predicted MW Explanation 59 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens tyrosine hydroxylase (TH), transcript variant 2
Alternative NameDYT14; DYT5b; TYH
Database LinkNP_000351
Entrez Gene 7054 Human
Entrez Gene 21823 Mouse
Entrez Gene 25085 Rat
FunctionThe protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq].
Related PathwayDruggable Genome Tyrosine metabolismMetabolic pathwaysParkinson's disease

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Tyrosine Hydroxylase antibody [EP1533Y]; Anti-Tyrosine Hydroxylase antibody [EP1533Y] at 1/500 dilution + human adrenal gland lysate at 10 ug.Secondary.Goat anti-rabbit HRP at 1/1000 dilution.Predicted band size : 59 kDa.Observed band size : 59 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1533Y]; IHC image ofTyrosine Hydroxylasestaining inhuman cerebral cortexformalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with TA303716, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody [EP1533Y]; ICC/IF image of TA303716 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody TA303716 at 1/100 dilution overnight at +4°C. The secondary antibody (green)was DyLight? 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
FC Image
Flow Cytometry-Anti-Tyrosine Hydroxylase antibody [EP1533Y](TA303716); Overlay histogram showing SH-SY5Y cells stained with TA303716 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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