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Anti-SRC PHOSPHO Antibody Y232
|A synthetic phospho-peptide corresponding to residues surrounding Tyrosine 529 of human Src was used as immunogen. The antibody will detect Src phosphorylation on Tyrosine 529 of both isoforms. Predicted to cross-react with mouse (both isoforms), rat and|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IF, DB
||WB: 1:5,000-10,000; ICC: 1:50; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
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Western blot - Src (phospho Y529) antibody [Y232]; All lanes : Anti-Src (phospho Y529) antibody [Y232] at 1/10000 dilution.Lane 1 : Untreated A431 cells.Lane 2 : EGF treated A431 cells.Lane 3 : EGF and alkaline phosphatase treated A431 cells.Predicted band size : 60 kDa.Observed band size : 55 kDa .
Immunocytochemistry/ Immunofluorescence - Src (phospho Y529) antibody [Y232]; Immunofluorescent analysis of Src (phospho Y529) expression in A431 cells using 1/50 TA303702.
Dot Blot - Src (phospho Y529) antibody [Y232]; Dot Blot analysis on immunogen phospho peptide (A) and non phospho peptide (B) using 1/2000 TA303702.