A synthetic phospho-peptide corresponding to residues surrounding Tyrosine 529 of human Src was used as immunogen. The antibody will detect Src phosphorylation on Tyrosine 529 of both isoforms. Predicted to cross-react with mouse (both isoforms), rat and
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].
EGFR1 Signaling Pathway
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Western blot analysis on A431 cell lysate using anti-Phospho-Src (pY529) RabMAb (TA303702), dilution 1:10,000. Cells were either (A) untreated, (B) treated with EGF and (C) treated with EGF+ alkaline phosphatase.