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Anti-ROCK1 Antibody EPR638Y
Also for ROCK1 (NM_005406)
|A synthetic peptide corresponding to residues near the C-terminus of human ROCK1 was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||ICC/IF: Use at an assay dependent concentration; WB: 1:500 - 1:1000; IP: 1:60; ICC: 1:50 - 1:100; IHC-P: 1:50 - 1:100
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Is unsuitable for Flow Cyt.
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Western blot - Anti-ROCK1 antibody [EPR638Y]; Anti-ROCK1 antibody [EPR638Y] at 1/500 dilution + HeLa cell lysate at 10 ug.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 158 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK1 antibody [EPR638Y]; Immunohistochemical analysis of paraffin embedded Human lymphoma tissue labelling ROCK1 with TA303689 at a dilution of 1/50.
Immunocytochemistry/ Immunofluorescence - Anti-ROCK1 antibody [EPR638Y]; TA303689 (1/200) staining ROCK1 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red).