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Anti-RAP1GAP Antibody Y134
Also for RAP1GAP (NM_002885)
|A synthetic peptide corresponding to residues following the GoLoco motif of human Rap1GAP was used as immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||ICC/IF: Use a concentration of 1 ug/ml; WB: 1:10000 - 1:50000; IHC-P: Use at an assay dependent dilution; ICC: 1:100 - 1:250; FC: 1:50; IP: 1:60
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|IgG fraction (Protein A or G Sepharose)
|Homo sapiens RAP1 GTPase activating protein (RAP1GAP), transcript variant 3|
|RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP|
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Western blot - RAP1GAP antibody [Y134]; Anti-RAP1GAP antibody [Y134] at 1/50000 dilution + Jurkat cell lysate.Predicted band size : 95 kDa.Observed band size : 95 kDa.
Immunohistochemistry (Paraffin-embedded sections) - RAP1GAP antibody [Y134]; Paraffin-embedded human breast cancer tissue.ab32373 at 1/100 dilution
Immunocytochemistry/ Immunofluorescence-RAP1GAP antibody [Y134](ab32373); ICC/IF image of ab32373 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was , DyLight? 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Flow Cytometry-Anti-RAP1GAP antibody [Y134](ab32373); Overlay histogram showing SH-SY5Y cells stained with ab32373 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.