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Home Antibody All anti-RAP1GAP antibodies

Anti-RAP1GAP Antibody Y134

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303681
  • Rabbit monoclonal antibody against Rap1Gap, N-Term(Y134)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419032) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for RAP1GAP (NM_002885)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues following the GoLoco motif of human Rap1GAP was used as immunogen.
Clone NameY134 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsICC/IF: Use a concentration of 1 ug/ml; WB: 1:10000 - 1:50000; IHC-P: Use at an assay dependent dilution; ICC: 1:100 - 1:250; FC: 1:50; IP: 1:60
Predicted MW Explanation 95 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction

Reference Data

Target NameHomo sapiens RAP1 GTPase activating protein (RAP1GAP), transcript variant 3
Alternative NameRAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP
Database LinkNP_002876
Entrez Gene 5909 Human
Entrez Gene 110351 Mouse
Entrez Gene 313644 Rat
Function
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - RAP1GAP antibody [Y134]; Anti-RAP1GAP antibody [Y134] at 1/50000 dilution + Jurkat cell lysate.Predicted band size : 95 kDa.Observed band size : 95 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - RAP1GAP antibody [Y134]; Paraffin-embedded human breast cancer tissue.TA303681 at 1/100 dilution
IF Image
Immunocytochemistry/ Immunofluorescence-RAP1GAP antibody [Y134](TA303681); ICC/IF image of TA303681 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was , DyLight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
FC Image
Flow Cytometry-Anti-RAP1GAP antibody [Y134](TA303681); Overlay histogram showing SH-SY5Y cells stained with TA303681 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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