OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-PTGS2 antibodies

Anti-PTGS2 Antibody EP1978Y

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303673
  • Rabbit monoclonal antibody against COX2 (EP1978Y )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC424429) , 20ug
100ul 325 In Stock
Add to Shopping Cart
WB(1)
IHC(1)
IF(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human COX-2 was used as an immunogen.
Clone NameEP1978Y IsotypeTissue culture supernatant
Species ReactivityMouse, Human (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:500; ICC: 1:100 - 1:250
BufferDoes not react with Rat. Is unsuitable for Flow Cyt,IHC-Fr,IHC-P or IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt,IHC-Fr,IHC-P or IP.

Reference Data

Target NameHomo sapiens prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2)
Alternative NameCOX-2; COX2; GRIPGHS; hCox-2; PGG/HS; PGHS-2; PHS-2
Database LinkNP_000954
FunctionProstaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - COX2 antibody [EP1978Y]; Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] at 1/500 dilution + Raw264.7 cell lysate (10ug) + LPS.Secondary.Goat anti-Rabbit HRP conjugated at 2000 .Predicted band size : 69 kDa.Observed band size : 72 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y]; TA303673 stainingCOX2 / Cyclooxygenase 2in Mouse14.5 dpctissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with TBS-Tand blocked with 1% BSA+ 1% FBS in TBS for2 hoursat room temperature; antigen retrieval was by heat mediation in Tris buffer, pH9. Samples were incubated with primary antibody (1/100 in blocking buffer) for 16 hours. An undilutedHRP-conjugated Goat anti-rabbit IgG polyclonalwas used as the secondary antibody.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y]; TA303673 staining HeLa cells by ICC/IF. The cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X100 in PBS. The cells were then stained with TA303673 at 1/200 in PBS for 1h at 22°C. An Alexa Fluro 488 goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody. Nuclei are stained in red with DAPI. TA303673 produces the expected cytoplasmic staining pattern.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title