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Anti-PTGS2 Antibody EP1978Y
Also for PTGS2 (NM_000963)
|A synthetic peptide corresponding to residues in human COX-2 was used as an immunogen.|
||Tissue culture supernatant
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:500; ICC: 1:100 - 1:250
|Does not react with Rat. Is unsuitable for Flow Cyt,IHC-Fr,IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,IHC-Fr,IHC-P or IP.
|Homo sapiens prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2)|
|COX-2; COX2; GRIPGHS; hCox-2; PGG/HS; PGHS-2; PHS-2|
|Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq]. |
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Western blot - COX2 antibody [EP1978Y]; Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] at 1/500 dilution + Raw264.7 cell lysate (10ug) + LPS.Secondary.Goat anti-Rabbit HRP conjugated at 2000 .Predicted band size : 69 kDa.Observed band size : 72 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y]; ab62331 stainingCOX2 / Cyclooxygenase 2in Mouse14.5 dpctissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with TBS-Tand blocked with 1% BSA+ 1% FBS in TBS for2 hoursat room temperature; antigen retrieval was by heat mediation in Tris buffer, pH9. Samples were incubated with primary antibody (1/100 in blocking buffer) for 16 hours. An undilutedHRP-conjugated Goat anti-rabbit IgG polyclonalwas used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y]; ab62331 staining HeLa cells by ICC/IF. The cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X100 in PBS. The cells were then stained with ab62331 at 1/200 in PBS for 1h at 22°C. An Alexa Fluro 488 goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody. Nuclei are stained in red with DAPI. ab62331 produces the expected cytoplasmic staining pattern.