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Home Antibody All anti-PTEN antibodies

Anti-PTEN Antibody Y184

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA303672 Rabbit monoclonal antibody against PTEN, C-term(Y184) 100ul $325 3-7 Days
LC424803 PTEN HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(1)
IF(2)
FC(1)
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the C-term of human PTEN was used as immunogen.
Clone NameY184 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF, FC Suggested DilutionsWB: 1:500; FC: 1:20; IP: 1:50; ICC/IF: Use at an assay dependent dilution
Predicted MW Explanation 47 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction

Reference Data

Target NameHomo sapiens phosphatase and tensin homolog (PTEN), transcript variant 1
Alternative Name10q23del; BZS; CWS1; DEC; GLM2; MHAM; MMAC1; PTEN1; TEP1
Database LinkNP_000305
Entrez Gene 5728 Human
Entrez Gene 19211 Mouse
Entrez Gene 50557 Rat
FunctionThis gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq].
Related PathwayPhosphataseDruggable Genome Inositol phosphate metabolismPhosphatidylinositol signaling systemp53 signaling pathwayFocal adhesionTight junctionPathways in cancerMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
Western blot - PTEN antibody [Y184]; Anti-PTEN antibody [Y184] at 1/500 dilution + MCF7 cell lysate.Predicted band size : 47 kDa.Observed band size : 54 kDa .
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IF Image
Immunocytochemistry/ Immunofluorescence - PTEN antibody [Y184]; TA303672 staining PTEN in human white blood cells by Immunocytochemistry/ Immunofluorescence. The cells were acetone fixed then blocked using 1% BSA for 1 hour at 25°C. Samples were then incubated with primary antibody at a 1/100 dilution for 2 hours at 25°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor? 488 (green) used at a 1/500 dilution. DAPI was used to stain the cell nuclei (blue).
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IF Image
Immunocytochemistry/ Immunofluorescence - PTEN antibody [Y184]; ICC/IF image of TA303672 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was DyLight 488 goat anti-rabbit IgG - H&L, pre-adsorbed used at a 1/250 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
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FC Image
Flow Cytometry-PTEN antibody [Y184](TA303672); Overlay histogram showing MCF-7 cells stained with TA303672 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
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