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Anti-PTEN Antibody Y184
Also for PTEN (NM_000314)
|A synthetic peptide corresponding to residues in the C-term of human PTEN was used as immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IF, FC
||WB: 1:500; FC: 1:20; IP: 1:50; ICC/IF: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
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Western blot - PTEN antibody [Y184]; Anti-PTEN antibody [Y184] at 1/500 dilution + MCF7 cell lysate.Predicted band size : 47 kDa.Observed band size : 54 kDa .
Immunocytochemistry/ Immunofluorescence - PTEN antibody [Y184]; TA303672 staining PTEN in human white blood cells by Immunocytochemistry/ Immunofluorescence. The cells were acetone fixed then blocked using 1% BSA for 1 hour at 25Â°C. Samples were then incubated with primary antibody at a 1/100 dilution for 2 hours at 25Â°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor? 488 (green) used at a 1/500 dilution. DAPI was used to stain the cell nuclei (blue).
Immunocytochemistry/ Immunofluorescence - PTEN antibody [Y184]; ICC/IF image of TA303672 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was DyLight 488 goat anti-rabbit IgG - H&L, pre-adsorbed used at a 1/250 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
Flow Cytometry-PTEN antibody [Y184](TA303672); Overlay histogram showing MCF-7 cells stained with TA303672 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.