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Anti-PTEN Antibody Y184
Also for PTEN (NM_000314)
|A synthetic peptide corresponding to residues in the C-term of human PTEN was used as immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IF, FC
||WB: 1:500; FC: 1:20; IP: 1:50; ICC/IF: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|IgG fraction (Protein A or G Sepharose)
|Homo sapiens phosphatase and tensin homolog (PTEN)|
|10q23del; BZS; CWS1; DEC; GLM2; MHAM; MMAC1; PTEN1; TEP1|
|This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq]. |
Senescence and Autophagy
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Western blot - PTEN antibody [Y184]; Anti-PTEN antibody [Y184] at 1/500 dilution + MCF7 cell lysate.Predicted band size : 47 kDa.Observed band size : 54 kDa .
Immunocytochemistry/ Immunofluorescence - PTEN antibody [Y184]; ab32199 staining PTEN in human white blood cells by Immunocytochemistry/ Immunofluorescence. The cells were acetone fixed then blocked using 1% BSA for 1 hour at 25°C. Samples were then incubated with primary antibody at a 1/100 dilution for 2 hours at 25°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor? 488 (green) used at a 1/500 dilution. DAPI was used to stain the cell nuclei (blue).
Immunocytochemistry/ Immunofluorescence - PTEN antibody [Y184]; ICC/IF image of ab32199 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was DyLight? 488 goat anti-rabbit IgG - H&L, pre-adsorbed used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Flow Cytometry-PTEN antibody [Y184](ab32199); Overlay histogram showing MCF-7 cells stained with ab32199 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.