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Anti-PRKCA PHOSPHO Antibody E195
Also for PRKCA (NM_002737)
|A synthetic phospho-peptide corresponding to residues surrounding Thr638 of human PKC? was used as immunogen. The antibody only detects PKC alpha phosphorylated on Threonine 638. Predicted to cross-react with rat, bovine and chicken, based on sequence hom|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1:20,000; IHC: 1:250; ICC: 1:500; IP: 1:40
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified
|Homo sapiens protein kinase C, alpha (PRKCA)|
|AAG6; PKC-alpha; PKCA; PRKACA|
Entrez Gene 5578 Human
Entrez Gene 18750 Mouse
Entrez Gene 24680 Rat
|Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq]. |
EGFR1 Signaling Pathway
MAPK signaling pathway
Wnt Signaling Pathway
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Western blot - PKC alpha (phospho T638) antibody [E195]; All lanes : Anti-PKC alpha (phospho T638) antibody [E195] at 1/20000 dilution.Lane 1 : 293 cell lysate, untreated.Lane 2 : 293 cell lysate, treated with TPA.Predicted band size : 77 kDa.Observed band size : 80 kDa .
Immunohistochemistry (Paraffin-embedded sections) - PKC alpha (phospho T638) antibody [E195]; Immunohistochemical staining of untreated paraffin-embedded normal brain tissue using TA303657 at 1/250 dilution.
Immunohistochemistry (Paraffin-embedded sections) - PKC alpha (phospho T638) antibody [E195]; Immunohistochemical staining of Phosphatase-treated paraffin-embedded normal brain tissue using TA303657 at 1/250 dilution