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Home Antibody All anti-PRKACA antibodies

Anti-PRKACA Antibody EP2102Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303655
  • Rabbit monoclonal antibody against PKA C alpha (EP2102Y )
  • FREE positive control: HEK293T cell transient overexpression lysate (LC419140) , 20ug
100ul $325 3-7 Days
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for PRKACA (NM_002730)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human PKC C-alpha subunit was used as immunogen.
Clone NameEP2102Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:50,000 - 200,000; IHC: 1:100; ICC: 1:100 - 250; FC: 1;60; IP: 1;50
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 1
Alternative NamePKACA
Database LinkNP_002721
FunctioncAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq].
Related Pathway
Cell cycle
Hedgehog signaling pathway
MAPK signaling pathway
Melanogenesis
Wnt Signaling Pathway

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WB Image
Western blot - cAMP Protein Kinase Catalytic subunit antibody [EP2102Y]; All lanes : Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] at 1/200000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : MCF-7 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/1000 dilution.Predicted band size : 46 kDa.Observed band size : 42 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - cAMP Protein Kinase Catalytic subunit antibody [EP2102Y]; TA303655, at a 1/100 dilution, staining human cAMP Protein Kinase Catalytic Subunit in testis by Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y]; ICC/IF image of TA303655 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
FC Image
Flow Cytometry-Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y](TA303655); Overlay histogram showing HeLa cells stained with TA303655 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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