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Anti-PRKAA1 Antibody Y365
Also for PRKAA1 (NM_006251)
|A synthetic peptide corresponding to residues near the C-term of human AMPK alpha-1 subunit was used as an immunogen|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||FC: 1:100; WB: 1:1000 - 1:5000; IHC-P: 1:100 - 1:250; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for ICC/IF.
|Homo sapiens protein kinase, AMP-activated, alpha 1 catalytic subunit (PRKAA1), transcript variant 1|
|The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]. |
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Western blot - AMPK alpha 1 antibody [Y365]; All lanes : Anti-AMPK alpha 1 antibody [Y365] at 1/5000 dilution.Lane 1 : MCF-7.Lane 2 : Hela.Predicted band size : 63 kDa.Observed band size : 63 kDa.
Immunohistochemistry (Paraffin-embedded sections) - AMPK alpha 1 antibody [Y365]; TA303654 at a 1:100 - 1:250 dilution staining AMPK alpha 1 in Human cervical carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.
Flow Cytometry - Anti-AMPK alpha 1 antibody [Y365]; Overlay histogram showing HeLa cells stained with TA303654 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.