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Anti-PPP3CA Antibody EP1669Y
Also for PPP3CA (NM_000944)
|A synthetic peptide corresponding to residues on the N-terminus of human PP2B was used as an immunogen.|
|Mouse, Rat, Hamster, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1:10000; IP: 1:100; FC: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for ICC or IHC-P.
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Western blot - Calcineurin A antibody [EP1669Y]; Anti-Calcineurin A antibody [EP1669Y] at 1/10000 dilution + Hela cell lysate at 10 ug.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 59 kDa.Observed band size : 59 kDa.
Flow Cytometry - Calcineurin A antibody [EP1669Y]; Overlay histogram showing HeLa cells stained with TA303653 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.