Home Antibody All anti-PIK3CG antibodies
Anti-PIK3CG Antibody Y388
Also for PIK3CG (NM_002649)
|A synthetic peptide of in the N-term of human PI3-K p110 gamma was used as an immunogen.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1,000; FC: 1:50; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified
|Does not react with Mouse, Rat
* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
Western blot - PI 3 Kinase catalytic subunit gamma antibody [Y388]; Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388] at 1/1000 dilution + Jurkat cell lysate.Predicted band size : 126 kDa.Observed band size : 110 kDa .
Flow Cytometry - Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388]; Overlay histogram showing HeLa cells stained with TA303640 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.