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Home Antibody All anti-PHB antibodies

Anti-PHB Antibody EP2803Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303635
  • Rabbit monoclonal antibody against Prohibitin (N-term) (EP2803Y )
  • FREE positive control: HEK293T cell transient overexpression lysate (LC419205) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(5)
FC(1)
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Also for PHB (NM_002634)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the N-terminus of human prohibitin was used as an immunogen.
Clone NameEP2803Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:10000 - 1:20000; IP: 1:60; IHC-P: 1:250 - 1:500; FC: 1:1000
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for ICC.

Reference Data

Target NameHomo sapiens prohibitin (PHB), transcript variant 2
Alternative NameHEL-215; HEL-S-54e; PHB1
Database LinkNP_002625
FunctionProhibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA. [provided by RefSeq].
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Prohibitin antibody [EP2803Y]; All lanes : Anti-Prohibitin antibody [EP2803Y] - Aminoterminal end at 1/20000 dilution.Lane 1 : MCF-7 cell lysate.Lane 2 : A431 cell lysate.Lane 3 : 293T cell lysate.Lane 4 : Ramos cell lysate.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 30 kDa.Observed band size : 30 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Prohibitin antibody [EP2803Y]; TA303635 at 1/250 dilution staining Prohibitin in human kidney by Immunohistochemistry, Paraffin-embedded tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Prohibitin antibody [EP2803Y]; TA303635 at 1/250 dilution staining Prohibitin in human hepatocellular carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Prohibitin antibody [EP2803Y] - Aminoterminal end(TA303635); TA303635 showing positive staining in Breast carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Prohibitin antibody [EP2803Y] - Aminoterminal end(TA303635); TA303635 showing positive staining in Normal liver tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Prohibitin antibody [EP2803Y] - Aminoterminal end(TA303635); TA303635 showing positive staining in Normal placenta tissue.
FC Image
Flow Cytometry - Anti-Prohibitin antibody [EP2803Y] - Aminoterminal end; Overlay histogram showing HepG2 cells stained with TA303635 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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