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Home Antibody All anti-PAK2 antibodies

Anti-PAK2 Antibody EP796Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303627
  • Rabbit monoclonal antibody against PAK 2 (EP796Y )
100ul 325 3-7 Days
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WB(1)
IHC(1)
FC(1)
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Also for PAK2 (NM_002577)
cDNA Clone shRNA/siRNA Primer Pair Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human PAK 2 was used as an immunogen.
Clone NameEP796Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:1000; IHC: 1:100; ICC: 1:100 - 250; FC: 1:100
Predicted MW Explanation 58 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens p21 protein (Cdc42/Rac)-activated kinase 2 (PAK2)
Alternative NamePAK65; PAKgamma
Database LinkNP_002568
Entrez Gene 5062 Human
Entrez Gene 224105 Mouse
Entrez Gene 29432 Rat
FunctionThe p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. [provided by RefSeq].
Related PathwayProtein KinaseDruggable Genome MAPK signaling pathwayErbB signaling pathwayAxon guidanceFocal adhesionT cell receptor signaling pathwayRegulation of actin cytoskeletonMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - PAK2 antibody [EP796Y]; Anti-PAK2 antibody [EP796Y] at 1/2000 dilution + HeLa cell lysate at 10 ug.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 58 kDa.Observed band size : 61 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAK2 antibody [EP796Y]; TA303627, at a 1/100 dilution, staining PAK2 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
FC Image
Flow Cytometry - Anti-PAK2 antibody [EP796Y]; Overlay histogram showing HeLa cells stained with TA303627 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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