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Anti-PAK1 PHOSPHO Antibody EP655Y
Also for PAK1 (NM_002576)
|A phosphor-specific peptide corresponding to residues surrounding Serine 20 on human PAK1 was used as an immunogen. This antibody recognized PAK1 phosphorylated at Ser20.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:2000; IHC: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens p21 protein (Cdc42/Rac)-activated kinase 1 (PAK1), transcript variant 2|
|PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternativelt spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. |
EGFR1 Signaling Pathway
MAPK signaling pathway
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Western blot - PAK1 (phospho S20) antibody [EP655Y]; All lanes : Anti-PAK1 (phospho S20) antibody [EP655Y] at 1/2000 dilution.Lane 1 : HeLa cell lysate, untreated.Lane 2 : HeLa cell lysate, treated with AP.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 61 kDa.Observed band size : 61 kDa.