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Anti-PAK1 Antibody EP656Y
Also for PAK1 (NM_002576)
|A synthetic peptide corresponding to residues in the human PAK1 protein was used as immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:10,000-50,000; IHC: 1: 250-500; ICC: 1:250-500; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
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Western blot - PAK1 antibody [EP656Y]; Anti-PAK1 (phospho S144) antibody [EP656Y] at 1/50000 dilution + HeLa cell lysate.Predicted band size : 65 kDa.Observed band size : 65 kDa.
Western blot - PAK1 (phospho S144) antibody [EP656Y]; All lanes : Anti-PAK1 (phospho S144) antibody [EP656Y].Lane 1 : HeLa Cell lysate.Lane 2 : HeLa Cell lysate treated with lambda phosphatase.Predicted band size : 65 kDa.Observed band size : 65 kDa.
Immunohistochemistry (Paraffin-embedded sections) - PAK1 (phospho S144) antibody [EP656Y]; Immunohistochemical analysis of paraffin-embedded human colon cancer using TA303623.
Immunocytochemistry/ Immunofluorescence - PAK1 (phospho S144) antibody [EP656Y]; Immunofluorescent staining of HeLa cells using TA303623.
Flow Cytometry - Anti-PAK1 (phospho S144) antibody [EP656Y]; Overlay histogram showing HeLa cells stained with TA303623 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.