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Anti-NFKBIA Antibody EP697
Also for NFKBIA (NM_020529)
|A synthetic peptide corresponding to residues on the C terminus of human IkappaB-alpha was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:500 -1000; IHC: 1:100 -250; ICC: 1:50 -100; FC: 1:70
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA)|
|IKBA; MAD-3; NFKBI|
|NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]. |
Delta-Notch Signaling Pathway
Toll-like receptor signaling pathway
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Western blot - IKB alpha antibody [EP697]; All lanes : Anti-IKB alpha antibody [EP697] at 1/1000 dilution.Lane 1 : HeLa cell lysates.Lane 2 : Jurkat cell lysates.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/1000 dilution.Predicted band size : 36 kDa.Observed band size : 36 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IKB alpha antibody [EP697]; ab76429 at 1/100 dilution staining IKB alpha in human kidneys by Immunohistochemistry, Paraffin-embedded tissue.
Flow Cytometry - Anti-IKB alpha antibody [EP697]; Overlay histogram showing HeLa cells stained with ab76429 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.