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Home Antibody All anti-NFE2L2 antibodies

Anti-NFE2L2 Antibody EP1808Y

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Specifications Citations (2) Related Products Product Documents
SKU Description Amount Price Availability*  
TA303616
  • Rabbit monoclonal antibody against Nrf2 (EP1808Y )
100ul 325 In Stock
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WB(1)
IHC(1)
IF(2)
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Also for NFE2L2 (NM_006164)
cDNA Clone shRNA/siRNA Primer Pair Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human Nrf2 was used as an immunogen.
Clone NameEP1808Y IsotypeTissue culture supernatant
Species ReactivityHuman (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsIHC-Fr: 1:100; ICC/IF: Use at an assay dependent dilution; WB: 1:1000 - 1:5000; IP: 1:20; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
BufferDoes not react with Rat. Is unsuitable for Flow Cyt.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens nuclear factor, erythroid 2-like 2 (NFE2L2), transcript variant 1
Alternative NameNRF2
Database LinkNP_006155
Entrez Gene 4780 Human
FunctionNFE2 (MIM 601490), NFE2L1 (MIM 163260), and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, such as JUN (MIM 165160) and FOS (MIM 164810), although remaining regions have diverged considerably from each other (Chan et al., 1995 [PubMed 7868116]).[supplied by OMIM].
Related PathwayTranscription Factors

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Nrf2 antibody [EP1808Y] - Carboxyterminal end; Anti-Nrf2 [EP1808Y] antibody at 1/2000 dilution + HepG2 cell lysate at 10 ug.Secondary.goat anti rabbit HRP at 1/2000 dilution.Predicted band size : 68 kDa.Observed band size : 100 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 antibody [EP1808Y] - Carboxyterminal end; Immunohistochemical analysis of Nrf2 expression in paraffin embedded human lung adenocarcinoma using 1/100 TA303616
IF Image
Immunocytochemistry/ Immunofluorescence - Nrf2 antibody [EP1808Y] - Carboxyterminal end; ICC/IF image of TA303616 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
IF Image
Immunocytochemistry/ Immunofluorescence - Nrf2 antibody [EP1808Y] - Carboxyterminal end; ICC/IF image of TA303616 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was DyLight 488 goat anti-rabbit IgG - H&L, pre-adsorbed used at a 1/250 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.

 

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