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Anti-NEDD8 Antibody Y297
Also for NEDD8 (NM_006156)
|A synthetic peptide corresponding to residues in the N-term of human NEDD8 was used as immunogen. This antibody detects NEDD8 bound to cullins proteins (neddylated cullins) as well as free NEDD8.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IP
||WB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250; IP: 1:50
|Does not react with Mouse. Is unsuitable for Flow Cyt.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens neural precursor cell expressed, developmentally down-regulated 8 (NEDD8)|
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Western blot - NEDD8 antibody [Y297]; All lanes : Anti-NEDD8 [Y297] antibody at 1/500 dilution.Lane 1 : HeLa cell lysate.Lane 2 : K562 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 9 kDa.Observed band size : 9 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NEDD8 antibody [Y297]; ab81264, at 1/100 dilution, staining NEDD8 in human lung carcinoma tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded section.
Immunoprecipitation - NEDD8 antibody [Y297]; Western blot analysis of immunoprecipitation pellet from HeLa cell lysate using ab81264 at 1/500 dilution. Band at 90 kDa shows Neddylated cullins, 50 kDa the IgG fraaction and 9 kDa Free NEDD8.