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Anti-MYC PHOSPHO Antibody E203
|A synthetic phospho-peptide corresponding to residues surrounding Thr58 and Ser62 of human C-Myc was used as immunogen. The antibody only detects c-Myc phosphorylated on Threonine 58 and Serine 62. Predicted to cross-react with most species, based on sequ|
||Lot dependent; please refer to CoA along with shipment
|WB, IF, FC
||WB: 1:1,000; ICC: 1:50-100; IP: 1:100
|49kDa (Detect a band at 57kDa)
|50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
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Western blot - c-Myc (phospho T58 + S62) antibody [E203]; All lanes : Anti-c-Myc (phospho T58 + S62) antibody [E203] at 1/1000 dilution.Lane 1 : Untreated A431 cell lysate.Lane 2 : TPA treated A431 cell lysate.Predicted band size : 49 kDa.Observed band size : 57 kDa .
Immunofluorescence - c-Myc (phospho T58 + S62) antibody [E203]; Immunofluorescent staining of A431 cells
Flow Cytometry - Anti-c-Myc (phospho T58 + S62) antibody; Overlay histogram showing Jurkat cells stained with TA303607 (red line). The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1:2000. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.