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Anti-MYC PHOSPHO Antibody E203
|A synthetic phospho-peptide corresponding to residues surrounding Thr58 and Ser62 of human C-Myc was used as immunogen. The antibody only detects c-Myc phosphorylated on Threonine 58 and Serine 62. Predicted to cross-react with most species, based on sequ|
||Lot dependent; please refer to CoA along with shipment
|WB, IF, FC
||WB: 1:1,000; ICC: 1:50-100; IP: 1:100
|50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
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Western blot - c-Myc (phospho T58 + S62) antibody [E203]; All lanes : Anti-c-Myc (phospho T58 + S62) antibody [E203] at 1/1000 dilution.Lane 1 : Untreated A431 cell lysate.Lane 2 : TPA treated A431 cell lysate.Predicted band size : 49 kDa.Observed band size : 57 kDa .
Immunofluorescence - c-Myc (phospho T58 + S62) antibody [E203]; Immunofluorescent staining of A431 cells
Flow Cytometry - Anti-c-Myc (phospho T58 + S62) antibody [E203]; Overlay histogram showing Jurkat cells stained with TA303607 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.