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Anti-MMP2 Antibody EP1183Y
Also for MMP2 (NM_004530)
|A synthetic peptide corresponding to residues on human MMP-2 was used as an immunogen. This antibody detects both the pro- and active forms of MMP-2.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000; FC: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for ICC/IF,IHC or IP.
|Homo sapiens matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) (MMP2), transcript variant 1|
|CLG4; CLG4A; MMP-II; MONA; TBE-1|
Entrez Gene 4313 Human
Entrez Gene 17390 Mouse
Entrez Gene 81686 Rat
|Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. |
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Western blot - MMP2 antibody [EP1183Y]; Anti-MMP2 antibody [EP1183Y] at 1/1000 dilution + Recombinant protein, 1ng.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 74 kDa.Observed band size : 72 kDa .
Flow Cytometry - Anti-MMP2 antibody [EP1183Y]; Overlay histogram showing MCF7 cells stained with TA303599 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.