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Home Antibody All anti-SMAD4 antibodies

Anti-SMAD4 Antibody EP618Y


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA303589 Rabbit monoclonal antibody against Smad4 (C-term)(EP618Y) 100ul $325 In Stock
LC401648 SMAD4 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the C-term of human Smad 4 was used as immunogen.
Clone NameEP618Y IsotypeRabbit IgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1,000-5,000; IHC: 1:100-250; ICC: 1:100-250; FC: 1:20; IP: 1:30
Predicted MW Explanation 65kDa
Buffer50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Smad4 antibody [EP618Y]; Anti-Smad4 antibody [EP618Y] at 1/5000 dilution + SHSY5Y cell lysate at 10 ug.Predicted band size : 65 kDa.

IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Smad4 antibody [EP618Y]; Immunohistochemical analysis of paraffin-embedded human lung carcinoma using TA303589 diluted 1:100

IHC Image
IHC - Anti-Smad4 antibody; TA303589 staining Smad4 in Rat femur tissue sections by IHC (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 20 minutes at 22°C; antigen retrieval was by heat mediation in a citrate buffer pH6.0. Samples were incubated with primary antibody (1/200 in blocking buffer) for 2 hours at 20°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Smad4 antibody [EP618Y]; TA303589 staining Smad4 in theA549 cell line from Human Lung by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde andpermeabilized with Triton X-100 0.25% in PBS. Samples were incubated with primary antibody (1/100) for 45 minutes at 25°C. A TRITC-conjugatedGoat anti-rabbit polyclonal (1/200)was used as the secondary antibody.

IF Image
Immunofluorescence - Smad4 antibody [EP618Y]; Immunofluorescent staining of HeLa cells using ab 40759 diluted 1:100

FC Image
Flow Cytometry - Smad4 antibody [EP618Y]; Flow cytometric analysis of permeabilized A431 cells using anti-Smad4 RabMAb (red) or a rabbit IgG (negative) (green).


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