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Anti-STMN1 Antibody Polyclonal
Also for STMN1 (NM_203399)
|A synthetic peptide corresponding to residues in N-terminus of human Stathmin was used as immunogen.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, IP
||WB: 1:50000; IP: Use a concentration of 5 ug/ml; FC: 1:15; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified
|Homo sapiens stathmin 1 (STMN1), transcript variant 2|
|C1orf215; Lag; LAP18; OP18; PP17; PP19; PR22; SMN|
|This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq]. |
MAPK signaling pathway
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Western blot - Stathmin 1 antibody; Anti-Stathmin 1 antibody at 1/50000 dilution + PC12 cell lysate at 10 µg.Predicted band size : 17 kDa.Observed band size : 17 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Stathmin 1 antibody; Immunohistochemical analysis of Stathmin 1 in paraffin embedded human tonsil tissue using TA303577 at 1/250 dilution.
Immunocytochemistry/ Immunofluorescence - Stathmin 1 antibody; Immunofluorescent staining of Stathmin 1 in HeLa cells using TA303577 at 1/100 dilution.
Immunoprecipitation - Anti-Stathmin 1 antibody; Stathmin 1 was immunoprecipitated using 0.5mg PC12 whole cell extract, 5ug of Rabbit polyclonal to Stathmin 1 and 50ul of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, PC12 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70Â°C; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with TA303577.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) .Band: 17kDa; Stathmin 1