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Home Antibody All anti-KRT10 antibodies

Anti-KRT10 Antibody EP1607IHCY

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA303572
  • Rabbit monoclonal antibody against Cytokeratin 10 (EP1607IHCY )
100ul $325 3-7 Days
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WB(1)
IHC(5)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the C-term of human Cytokeratin 10 was used as immunogen.
Clone NameEP1607IHCY IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsIHC-Fr: Use at an assay dependent concentration; WB: 1:50000 - 1:100000; IP: 1:30; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
Predicted MW Explanation 60 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens keratin 10, type I (KRT10)
Alternative NameBCIE; BIE; CK10; EHK; K10; KPP
Database LinkNP_000412
Entrez Gene 3858 Human
Entrez Gene 16661 Mouse
Entrez Gene 450225 Rat
FunctionThis gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Cytokeratin 10 antibody [EP1607IHCY]; Anti-Cytokeratin 10 antibody [EP1607IHCY] at 1/100000 dilution + HaCat cell lysate at 10 ug.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 60 kDa.Observed band size : 60 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 10 antibody [EP1607IHCY]; TA303572, at 1/100-1/250 dilution, staining Cytokeratin 10 in paraffin-embedded human skin tissue by Immunohistochemistry. Note positive staining of suprabasal layers of keratinising stratified epithelia.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY]; Fluorescent immunohistochemical analysis of paraffin-embedded human normal skin tissue using TA303572. Green-CK10 red-PI.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY]; IHC-P image of Cytokerati 10 staining on human skin section using TA303572 (1:6000). The sections were subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA for 10 mins at 21°C. TA303572 was incubated for 2 hours at 21°C. The secondary antibody used was Goat polyclonal to rabbit IgG conjugated to biotin (1:200).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY]; IHC-P image of Cytokerati 10 staining on rat skin section using TA303572 (1:10000). The sections were subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA for 10 mins at 21°C. TA303572 was incubated for 2 hours at 21°C. The secondary antibody used was Goat polyclonal to rabbit IgG conjugated to biotin (1:250).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY]; IHC-P image of Cytokerati 10 staining on mouse skin section using TA303572 (1:10000). The sections were subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA for 10 mins at 21°C. TA303572 was incubated for 2 hours at 21°C. The secondary antibody used was Goat polyclonal to rabbit IgG conjugated to biotin (1:250).
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