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Home Antibody All anti-STMN1 antibodies

Anti-STMN1 Antibody EP1573Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303554
  • Rabbit monoclonal antibody against Stathmin (EP1573Y )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC404338) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IP(1)
IHC(1)
IF(1)
FC(1)
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Also for STMN1 (NM_203399)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in C-terminus of human Stathmin was used as immunogen.
Clone NameEP1573Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, IP, FC Suggested DilutionsWB: 1:50000; IP: 1:50; ICC: 1:100 - 1:250; FC: 1:100; IHC-P: Use at an assay dependent dilution
Predicted MW Explanation 19kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens stathmin 1 (STMN1), transcript variant 2
Alternative NameC1orf215; Lag; LAP18; OP18; PP17; PP19; PR22; SMN
Database LinkNP_981944
Entrez Gene 3925 Human
Entrez Gene 16765 Mouse
Entrez Gene 29332 Rat
FunctionThis gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq].
Related Pathway MAPK signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Stathmin 1 antibody [EP1573Y]; Anti-Stathmin 1 antibody [EP1573Y] at 1/500000 dilution + PC12 lysate at 10 ug.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Observed band size : 18 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Stathmin 1 antibody [EP1573Y]; TA303554, at a 1/250 dilution, staining human Stathmin 1 in lymph node tissue, using Immunohistochemistry, Paraffin embedded sections.
IF Image
Immunocytochemistry/ Immunofluorescence - Stathmin 1 antibody [EP1573Y]; TA303554 stained HeLa cells
IP Image
Immunoprecipitation - Stathmin 1 antibody [EP1573Y]; Whole cell lysate prepared from human colon cells was loaded at 20ug. The immunoprecipitation step was performed using Protein A/G. TA303554 used at a 1/200 dilution for 12 hours at 4°C. For WB TA303554 used at a 1/10000 dilution.
FC Image
Flow Cytometry-Stathmin 1 antibody [EP1573Y](TA303554); Overlay histogram showing Jurkat cells stained with TA303554 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

 

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