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Anti-IL1R1 Antibody EP409Y
Also for IL1R1 (NM_000877)
|A synthetic peptide corresponding to residues near the N-terminus of human IL-1R type 1 was used as immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IF, FC
||WB: 1:1000 - 1:2000; FC: 1:10 - 1:50; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for ICC or IHC.
|Homo sapiens interleukin 1 receptor, type I (IL1R1), transcript variant 1|
|CD121A; D2S1473; IL-1R-alpha; IL1R; IL1RA; P80|
|The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA). It is an important mediator involved in many cytokine induced immune and inflammatory responses. This gene along with interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 2 (IL1RL2), and interleukin 1 receptor-like 1 (IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. [provided by RefSeq]. |
Cytokine-cytokine receptor interaction
Hematopoietic cell lineage
MAPK signaling pathway
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Western blot - IL1 Receptor I antibody [EP409Y]; Anti-IL1 Receptor I antibody [EP409Y] at 1/2000 dilution + Jurkat cell lysate at 10 µg.Predicted band size : 80 kDa.Observed band size : 75 kDa .
Immunocytochemistry/ Immunofluorescence - Anti-IL1 Receptor I antibody [EP409Y]; Immunofluorescence analysis of Human synovial fibroblasts, staining IL1 Receptor I with TA303544. Cells were fixed with formaldehyde and blocked with 2% fetal calf serum. Samples were incubated with primary antibody overnight and an AlexaFluor?594-conjugated anti-rabbit IgG was used as the secondary antibody.
Flow Cytometry - IL1 Receptor I antibody [EP409Y]; Overlay histogram showing Jurkat cells stained with TA303544 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22Â°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22Â°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1Âµg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a minimal signal in Jurkat cells fixed with methanol (5 min) used under the same conditions.Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.