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Anti-HMGB3 Antibody EP2839Y
Also for HMGB3 (NM_005342)
|A synthetic peptide corresponding to residues near the C-terminus of human Hmgb3 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:2500 - 5000; IHC: 1:100 - 250; FC: 1:20; IP: 1:40
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens high mobility group box 3 (HMGB3)|
|HMG-2a; HMG-4; HMG2A; HMG4|
|HMGB3 belongs to the high mobility group (HMG) protein superfamily. Like HMG1 (MIM 163905) and HMG2 (MIM 163906), HMGB3 contains DNA-binding HMG box domains and is classified into the HMG box subfamily. Members of the HMG box subfamily are thought to play a fundamental role in DNA replication, nucleosome assembly and transcription (Wilke et al., 1997 [PubMed 9370291]; Nemeth et al., 2006 [PubMed 16945912]).[supplied by OMIM]. COMPLETENESS: complete on the 3' end. |
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Western blot - HMG4 antibody [EP2839Y]; All lanes : Anti-HMG4 antibody [EP2839Y] at 1/5000 dilution.Lane 1 : 293T cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : human placenta cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti rabbit at 1/2000 dilution.Predicted band size : 23 kDa.Observed band size : 25 kDa .Additional bands at : 55 kDa (possible this band results from an immunoprecipitated sample, it is the ~55kDa heavy chain of the antibody used for the IP).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HMG4 antibody [EP2839Y]; Immunohistochemical analysis of paraffin-embedded human placenta using ab75782 at 1/100-1/250 dilution and with the antigen retrieval step.