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Anti-HMGB3 Antibody EP2839Y


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SKU Description Amount Price Availability*  
  • Rabbit monoclonal antibody against Hmgb3 (EP2839Y )
100ul $325 3-7 Days
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human Hmgb3 was used as an immunogen.
Clone NameEP2839Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:2500 - 5000; IHC: 1:100 - 250; FC: 1:20; IP: 1:40
Predicted MW Explanation 23 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens high mobility group box 3 (HMGB3), transcript variant 2
Alternative NameHMG-2a; HMG-4; HMG2A; HMG4
Database LinkNP_005333
Entrez Gene 3149 Human
Entrez Gene 15354 Mouse
Entrez Gene 305373 Rat
FunctionHMGB3 belongs to the high mobility group (HMG) protein superfamily. Like HMG1 (MIM 163905) and HMG2 (MIM 163906), HMGB3 contains DNA-binding HMG box domains and is classified into the HMG box subfamily. Members of the HMG box subfamily are thought to play a fundamental role in DNA replication, nucleosome assembly and transcription (Wilke et al., 1997 [PubMed 9370291]; Nemeth et al., 2006 [PubMed 16945912]).[supplied by OMIM]. COMPLETENESS: complete on the 3' end.
Related PathwayTranscription Factors

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - HMG4 antibody [EP2839Y]; All lanes : Anti-HMG4 antibody [EP2839Y] at 1/5000 dilution.Lane 1 : 293T cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : human placenta cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti rabbit at 1/2000 dilution.Predicted band size : 23 kDa.Observed band size : 25 kDa .Additional bands at : 55 kDa (possible this band results from an immunoprecipitated sample, it is the ~55kDa heavy chain of the antibody used for the IP).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HMG4 antibody [EP2839Y]; Immunohistochemical analysis of paraffin-embedded human placenta using TA303538 at 1/100-1/250 dilution and with the antigen retrieval step.


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