A synthetic peptide corresponding to residues near the C-terminus of human HIF-1 alpha was used as an immunogen.
Buffer
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Clone Name
EP1215Y
Isotype
Rabbit IgG
Species Reactivity
Human, Rat
Concentration
Purification
Guaranteed Application *
WB, IHC
Suggested Dilutions
WB: 1:2000 IHC: 1:100 250 ICC: 1:100 250
Background
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq].
Related Pathway
Adipogenesis
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