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Home Antibody All anti-GOLGA2 antibodies

Anti-GOLGA2 Antibody EP892Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303523
  • Rabbit monoclonal antibody against Golgi Matrix Protein (GM130)(EP892U)
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(4)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-term of human Golgi Matrix Protein (GM130) was used as a immunogen.
Clone NameEP892Y IsotypeIgG
Species ReactivityMouse, Rat, Cow, Dog, Human, African Green Monkey ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsICC/IF: 1:100 - 1:250; IHC-P: 1:250 - 1:500; IHC-Fr: Use at an assay dependent dilution; WB: 1:1000 - 1:10000; IP: 1:50
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens golgin A2 (GOLGA2)
Alternative NameGM130
Database LinkNP_004477
Entrez Gene 2801 Human
Entrez Gene 99412 Mouse
Entrez Gene 64528 Rat
Entrez Gene 717677 Monkey
Entrez Gene 480708 Dog
FunctionThe Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. The golgins are a family of proteins, of which the protein encoded by this gene is a member, that are localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - GM130 antibody [EP892Y]; Anti-GM130 antibody [EP892Y] at 1/200000 dilution + 10 ug of HeLa cell lysate.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 112 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - GM130 antibody [EP892Y]; TA303524 (1/500) staining GM130 in paraffin-embedded Human liver tissue sections.
IF Image
Immunocytochemistry/ Immunofluorescence - GM130 antibody [EP892Y]; TA303524 staining GM130 in human ARPE-19 cells by ICC/IF (immunocytochemistry/immunofluorescence). Cells were formaldehyde fixed, permeabilized by 0.5% TX-100 and blocked with 5% serum for 20 minutes at 25°C. The sample was incubated with the primary antibody (1/500 in 1% goat serum, 0.1%TX100, 1 x PBS) for 16 hours at 4°C. An Alexa Fluor?488-conjugated Goat anti-rabbit polyclonal (1/500) was used as the secondary.
IF Image
Immunocytochemistry/ Immunofluorescence - GM130 antibody [EP892Y]; TA303524 staining GM130 in Bovine brain microvascular endothelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% saponin and blocked with 5% BSA for 90 minutes at 37°C. Samples were incubated with primary antibody (1/100 in 0.1% saponin + 1% BSA ) for 18 hours at 4°C. An undiluted Alexa Fluor?568-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
IF Image
Immunocytochemistry/ Immunofluorescence - GM130 antibody [EP892Y]; ICC/IF image of stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody , 1ug/ml) overnight at +4°C. The secondary antibody (green) was DyLight 488 goat anti-rabbit IgG - H&L, pre-adsorbed used at a 1/250 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
IF Image
Immunocytochemistry/ Immunofluorescence - GM130 antibody [EP892Y]; ICC/IF image of TA303524 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.

 

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