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Home Antibody All anti-ESRRA antibodies

Anti-ESRRA Antibody EPR46Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303506
  • Rabbit monoclonal antibody against Estrogen Related Receptor alpha (EPR46Y )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC417978) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IP(1)
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Also for ESRRA (NM_004451)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human ERRalpha was used as an immunogen.
Clone NameEPR46Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IP Suggested DilutionsWB: 1:1000 - 1:10000; IP: Use at an assay dependent concentration; FC: 1:20
Predicted MW Explanation 55 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for ICC or IHC-P.

Reference Data

Target NameHomo sapiens estrogen-related receptor alpha (ESRRA), transcript variant 1
Alternative NameERR1; ERRa; ERRalpha; ESRL1; NR3B1
Database LinkNP_004442
Entrez Gene 2101 Human
Entrez Gene 26379 Mouse
Entrez Gene 293701 Rat
FunctionThe protein encoded by this gene is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site-specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium-chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes. A processed pseudogene of ESRRA is located on chromosome 13q12.1. [provided by RefSeq].
Related PathwayNuclear Hormone ReceptorTranscription FactorsDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Estrogen Related Receptor alpha antibody [EPR46Y]; Anti-Estrogen Related Receptor alpha antibody [EPR46Y] at 1/5000 dilution + HeLa cell lysate at 10 ug.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 55 kDa.Observed band size : 55 kDa.
IP Image
Immunoprecipitation - Anti-Estrogen Related Receptor alpha antibody [EPR46Y]; Estrogen Related Receptor alpha was immunoprecipitated using 0.5mg Hela whole cell extract, 10ug of Rabbit monoclonal to Estrogen Related Receptor alpha and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). .The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70oC; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with TA303506.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) .Band: Band: 55kDa: Estrogen Related Receptor alpha.

 

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