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Anti-EGFR PHOSPHO Antibody EPR2149Y
Also for EGFR (NM_005228)
|A phospho specific peptide corresponding to residues surrounding tyrosine 845 of human EGFR was used as an immunogen. This antibody detects EGFR phosphorylated on tyrosine 845|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:500; ICC/IF: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,IHC-P or IP.
|Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 1|
|ERBB; ERBB1; HER1; mENA; PIG61|
|The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]. |
Cytokine-cytokine receptor interaction
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
MAPK signaling pathway
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Western blot - Anti-EGFR (phospho Y845) antibody; All lanes : Anti-EGFR (phospho Y845) [EPR2149Y] antibody at 1/500 dilution.Lane 1 : A431 cell lysates, untreated .Lane 2 : A431 cell lysates, treated with EGF.Lysates/proteins at 10 µg per lane.Predicted band size : 134 kDa.
Immunocytochemistry/ Immunofluorescence - EGFR (phospho Y845) antibody; ab97613 at 1/100 dilution staining EGFR in A431 by Immunofluorescence.