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Anti-EGFR PHOSPHO Antibody EP2259Y
Also for EGFR (NM_005228)
|A synthetic phospho-peptide corresponding to residues surrounding Serine 1070 and 1071 of human EGF receptor was used as immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:500000 - 1:1e+006; IP: 1:30; ICC/IF: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt or IHC-P.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IHC-P.
|Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 1|
|ERBB; ERBB1; HER1; mENA; PIG61|
|The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]. |
Cytokine-cytokine receptor interaction
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
MAPK signaling pathway
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Western blot - EGFR (phospho S1070 + S1071) antibody [EP2259IHCY]; All lanes : Anti-EGFR (phospho S1070 + S1071) antibody [EP2259Y] at 1/1000000 dilution.Lane 1 : A431 cell lysate.Lane 2 : A431 cell lysate treated with EGF.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 134 kDa.Observed band size : 180 kDa .
Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho S1070 + S1071) antibody [EP2259IHCY]; Immunofluorescence staining of A431 cells using ab76300 at 1/100-1/250 dilution.