OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-EGFR antibodies

Anti-EGFR PHOSPHO Antibody EP38Y

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303488
  • Rabbit monoclonal antibody against EGF Receptor (Pan) (EP38Y ) (phospho-specific)
100ul 325 3-7 Days
Add to Shopping Cart
WB(1)
IHC(6)
FC(1)
Assay(1)
spacer
Also for EGFR (NM_005228)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Tyr1068 of human EGF receptor was used as immunogen. The antibody detects both EGFR phosphorylated on Tyrosine 1068 of the mature human isoform 1 (corresponding to Y1092 from the precursor
Clone NameEP38Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC, FC Suggested DilutionsWB: 1:100000; IP: 1:50; FC: 1:100; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:250 - 1:500
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens epidermal growth factor receptor (EGFR), transcript variant 1
Alternative NameERBB; ERBB1; HER1; mENA; PIG61
Database LinkNP_005219
Entrez Gene 1956 Human
Entrez Gene 13649 Mouse
Entrez Gene 24329 Rat
FunctionThe protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq].
Related Pathway
Adherens junction
Cytokine-cytokine receptor interaction
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
Focal Adhesion
MAPK signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - EGFR antibody [EP38Y]; Anti-EGFR antibody [EP38Y] at 1/100000 dilution + HeLa cell lysate at 10 ug.Secondary.Goat anti-Rabbit HRP labeled. at 1/2000 dilution.Predicted band size : 134 kDa.Observed band size : 175 kDa .
Assay Image
Other-Anti-EGFR antibody [EP38Y](TA303488); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - EGFR antibody [EP38Y]; Ab52894 (1:100) staining human EGFR in human lymph node metastasis tissue by immunohistochemistry using paraffin embedded tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-EGFR antibody [EP38Y](TA303488); TA303488 showing positive staining in Cervical carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-EGFR antibody [EP38Y](TA303488); TA303488 showing positive staining in Endometrial carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-EGFR antibody [EP38Y](TA303488); TA303488 showing positive staining in Glioma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-EGFR antibody [EP38Y](TA303488); TA303488 showing positive staining in Normal tonsil squamous cells tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-EGFR antibody [EP38Y](TA303488); TA303488 showing positive staining in Renal cell carcinoma tissue.
FC Image
Flow Cytometry - Anti-EGFR antibody [EP38Y]; Overlay histogram showing A431 cells stained with TA303488 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title