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Anti-EGFR PHOSPHO Antibody Y39
Also for EGFR (NM_005228)
|A synthetic phospho-peptide corresponding to residues surrounding Tyr1086 of human EGF receptor was used as immunogen. The antibody only detects EGFR phosphorylated on Tyrosine 1086 of the mature human isoform 1 (corresponding to Y1110 from the precursor|
|Human (Does not react with: Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:5000; ICC: 1:100 - 1:250; IP: 1:150
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for Flow Cyt or IHC.
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Western blot - EGFR (phospho Y1086) antibody [Y39]; All lanes : Anti-EGFR (phospho Y1086) antibody [Y39] at 1/10000 dilution.Lane 1 : Untreated A431 cell lysate.Lane 2 : A431 cell lysate treated with EGF.Predicted band size : 134 kDa.Observed band size : 150 kDa .
Immunofluorescence - EGFR (phospho Y1086) antibody [Y39]; Immunofluorescent analysis of EGFR (phospho Y1086) expression in A431 cell culture, using 1/100 TA303486.