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Anti-EGFR PHOSPHO Antibody Y39
Also for EGFR (NM_005228)
|A synthetic phospho-peptide corresponding to residues surrounding Tyr1086 of human EGF receptor was used as immunogen. The antibody only detects EGFR phosphorylated on Tyrosine 1086 of the mature human isoform 1 (corresponding to Y1110 from the precursor|
||Whole Cell Lysate
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:5000; ICC: 1:100 - 1:250; IP: 1:150
|Does not react with Rat. Is unsuitable for Flow Cyt or IHC.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IHC.
|Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 1|
|ERBB; ERBB1; HER1; mENA; PIG61|
|The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]. |
Cytokine-cytokine receptor interaction
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
MAPK signaling pathway
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Western blot - EGFR (phospho Y1086) antibody [Y39]; All lanes : Anti-EGFR (phospho Y1086) antibody [Y39] at 1/10000 dilution.Lane 1 : Untreated A431 cell lysate.Lane 2 : A431 cell lysate treated with EGF.Predicted band size : 134 kDa.Observed band size : 150 kDa .
Immunofluorescence - EGFR (phospho Y1086) antibody [Y39]; Immunofluorescent analysis of EGFR (phospho Y1086) expression in A431 cell culture, using 1/100 ab32086.