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Home Antibody All anti-DNTT antibodies

Anti-DNTT Antibody EPR2976Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303481
  • Rabbit monoclonal antibody against TDT (EPR2976Y )
  • FREE positive control: HEK293T cell transient overexpression lysate (LC422737) , 20ug
100ul 325 3-7 Days
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WB(1)
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Also for DNTT (NM_001017520)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the C terminus of human TDT was used as an immunogen.
Clone NameEPR2976Y IsotypeTissue culture supernatant
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB Suggested DilutionsWB: 1:10000 - 1:50000; IP: 1:20; ICC/IF: 1:100 - 1:250
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt or IHC-P.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt or IHC-P.

Reference Data

Target NameHomo sapiens DNA nucleotidylexotransferase (DNTT), transcript variant 2
Alternative NameTDT
Database LinkNP_001017520
FunctionThis gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq].
Related Pathway
Hematopoietic cell lineage

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WB Image
Western blot - TdT antibody [EPR2976Y]; All lanes : Anti-TdT antibody [EPR2976Y] at 1/10000 dilution.Lane 1 : Human thymus lysate.Lane 2 : Jurkat lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 58 kDa.Observed band size : 58 kDa.Additional bands at : 24 kDa,35 kDa. We are unsure as to the identity of these extra bands.

 

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