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Anti-CTNNB1 Antibody E247
Also for CTNNB1 (NM_001904)
|A synthetic peptide corresponding to residues near N-terminus of human ?-catenin was used as immunogen.|
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:5,000-10,000; IHC: 1:500; ICC: 1:250; IP: 1:100
|50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Homo sapiens catenin (cadherin-associated protein), beta 1, 88kDa (CTNNB1), transcript variant 1|
|armadillo; CTNNB; MRD19|
|Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM]. |
TGF Beta Signaling Pathway
Wnt Signaling Pathway
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Western blot image of TA303471 staining whole cell lysate of U2OS cells. The gel was blocked with 5% milk for 1 hour at 21°C. The primary antibody was diluted 1/5000 and incubated for 12 hours at 4°C. A HRP conjugated swine anti-rabbit antibody was used as the secondary.
TA303471 staining beta Catenin in Dog colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in 10mM citrate buffer, pH6. Samples were incubated with primary antibody (1/250 in PBS with 1x casein) for 90 minutes at 25°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
TA303471, at a 1/500 dilution, staining beta Catenin in paraffin embedded human colon adenocarcinoma tissue sections by Immunohistochemistry.
TA303471 at 1/200 staining mouse small intestine tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed before incubation with the primary antibody. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
TA303471 staining human renal carcinoma tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking with 1% milk for 45 minutes at 22°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 22°C. A HRP conjugated goat anti-rabbit antibody, diluted 1/400, was used as the secondary.
TA303471 showing positive staining in Cervical carcinoma tissue (formalin/PFA-fixed paraffin-embedded sections)
TA303471 showing positive staining in Breast carcinoma tissue (formalin/PFA-fixed paraffin-embedded sections)
TA303471 showing positive staining in Lung adenocarcinoma tissue (formalin/PFA-fixed paraffin-embedded sections)
TA303471 showing positive staining in Papillary carcinoma of thyroid gland tissue (formalin/PFA-fixed paraffin-embedded sections)
TA303471 showing positive staining in Kidney carcinoma tissue (formalin/PFA-fixed paraffin-embedded sections)
TA303471 staining ßcatenin in SW480 cells treated with BIO, by ICC/IF. Increase of ßcatenin expression correlates with increased concentration of BIO, as described in literature. The cells were incubated at 37°C for 48h in media containing different concentrations of BIO in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA303471 (1/200) dilution was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
TA303471 at a 1/250 dilution, staining beta Catenin in A431 cells by Immunofluorescence.
TA303471 staining beta Catenin in mouse liver tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with formaldehyde and blocked with 5% serum at 40C for 30 minutes. The sample was incubated with primary antibody (1/200) in dilution buffer containing PBS and 3% Goat Serum at 40C for 9 hours. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at 1/200 dilution.