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Anti-CRKL PHOSPHO Antibody EP270Y
Also for CRKL (NM_005207)
|A synthetic phospho-peptide corresponding to residues surrounding Tyr207 of human CrkL was used as immunogen. The antibody only detects CrkL phosphorylated on Tyrosine 207.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:50000; IP: 1:50
|Does not react with Rat. Is unsuitable for Flow Cyt or IHC-P.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IHC-P.
|Homo sapiens v-crk avian sarcoma virus CT10 oncogene homolog-like (CRKL)|
|v-crk avian sarcoma virus CT10 oncogene homolog-like; v-crk sarcoma virus CT10 oncogene homolog (avian)-like|
|This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic. |
EGFR1 Signaling Pathway
MAPK signaling pathway
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Western blot - CrkL antibody [EP270Y]; All lanes : Anti-CrkL (phospho Y207) antibody [EP270Y] at 1/50000 dilution.Lane 1 : (A) K562 cell lysates untreated.Lane 2 : (B) K562 cell lysates treated with AP.Lysates/proteins at 10 µg per lane.Predicted band size : 34 kDa.Observed band size : 37 kDa .