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Anti-AP2M1 Antibody EP2695Y
Also for AP2M1 (NM_001025205)
|A synthetic peptide corresponding to residues near the C-terminus of human AP2 mu1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:5000; ICC: 1:100 - 1:250; FC: 1:60
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IHC-P or IP.
|Homo sapiens adaptor-related protein complex 2, mu 1 subunit (AP2M1), transcript variant 2|
|AP50; CLAPM1; mu2|
|This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. |
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Western blot - AP2M1 antibody [EP2695Y]; All lanes : Anti-AP2M1 antibody [EP2695Y] at 1/1000 dilution.Lane 1 : MCF-7 cell lysate.Lane 2 : SKBR-3 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti rabbit at 1/2000 dilution.Predicted band size : 50 kDa.Observed band size : 50 kDa.
Flow Cytometry - AP2M1 antibody [EP2695Y]; Flow cytometric analysis of AP2M1 in permeabilized MCF-7 cells using ab75995 (red) at 1/60 dilution or a rabbit IgG antibody (negative) (green).