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Anti-CHUK Antibody Y463
Also for CHUK (NM_001278)
|A synthetic peptide corresponding to residues in the N-term of human IKK-alpha was used as immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||ICC: 1:500; WB: 1:10000 - 1:50000; IHC-P: Use at an assay dependent dilution; FC: 1:50; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
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Western blot - IKK alpha antibody [Y463]; Anti-IKK alpha antibody [Y463] at 1/50000 dilution + Daudi cell lysate.Predicted band size : 85 kDa.Observed band size : 88 kDa .
Western blot - Anti-IKK alpha antibody [Y463]; Anti-IKK alpha antibody [Y463] at 1/10000 dilution + Active human IKK alpha full length protein at 0.01 ug.Secondary.Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution.developed using the ECL technique.Performed under reducing conditions.Exposure time : 10 seconds
Immunohistochemistry (Paraffin-embedded sections) - IKK alpha antibody [Y463]; Ab32041, at a dilution of 1/50, staining IKK alpha in paraffin embedded human stomach carcinoma by Immunohistochemisty.
Flow Cytometry - IKK alpha antibody [Y463]; Overlay histogram showing HeLa cells stained with TA303452 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.