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Anti-CEBPA Antibody EP708Y
Also for CEBPA (NM_004364)
|A synthetic peptide corresponding to residues in the N-term of human C/EBP alpha was used as immunogen. This antibody recognizes both the 42kDa and the 30kDa isoforms.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IF, FC
||WB: 1:1000 - 1:10000; ICC/IF: 1:250 - 1:500; FC: 1:100
|Does not react with Mouse, Rat. Is unsuitable for IHC or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IHC or IP.
|Homo sapiens CCAAT/enhancer binding protein (C/EBP), alpha (CEBPA), transcript variant 1|
|The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. [provided by RefSeq]. |
EGFR1 Signaling Pathway
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Western blot - CEBP Alpha antibody [EP708Y]; Anti-CEBP Alpha antibody [EP708Y] at 1/1000 dilution + U937 + LPS cell lysate at 10 µg.Predicted band size : 30,43 kDa.Observed band size : 30,43 kDa.
Western blot - Anti-CEBP Alpha antibody [EP708Y]; Anti-CEBP Alpha antibody [EP708Y] at 1/5000 dilution + Human MCF-7 whole cell lysate at 100000 cells.Secondary.HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/2000 dilution.developed using the ECL technique.Performed under reducing conditions.Predicted band size : 30,43 kDa.Observed band size : 40 kDa .Exposure time : 1 minuteThis image is courtesy of an anonymous Abreview
Immunocytochemistry/ Immunofluorescence - CEBP Alpha antibody [EP708Y]; ab40761 (1/250) staining HeLa cells using immunofluorescence.
Flow Cytometry - CEBP Alpha antibody [EP708Y]; Overlay histogram showing HeLa cells stained with ab40761 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.