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Anti-CDKN1C Antibody EP2515Y
Also for CDKN1C (NM_000076)
|A synthetic peptide corresponding to residues near the N-terminus of human p57 KIP2 was used as an immunogen.|
|HUman, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:500; IP: 1:20; IHC-P: 1:100 - 1:250; ICC: 1:100; FC: 1:30
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens cyclin-dependent kinase inhibitor 1C (p57, Kip2) (CDKN1C), transcript variant 1|
|BWCR; BWS; KIP2; p57; p57Kip2; WBS|
|The protein encoded by this gene is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]. |
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Western blot - p57 Kip2 antibody [EP2515Y]; All lanes : Anti-p57 Kip2 antibody [EP2515Y] at 1/500 dilution.Lane 1 : HeLa lysate.Lane 2 : Jurkat lysate.Lane 3 : human brain lysate.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 32 kDa.Observed band size : 57 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - p57 Kip2 antibody [EP2515Y]; TA303447 at 1/100 dilution staining p57 Kip2 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - p57 Kip2 antibody [EP2515Y]; TA303447 at 1/100 dilution staining p57 Kip2 in human placenta by Immunohistochemistry, Paraffin-embedded tissue.
Immunocytochemistry/ Immunofluorescence - Anti-p57 Kip2 antibody [EP2515Y]; TA303447 staining p57 Kip2 in HeLa cells treated with dexamethasone , by ICC/IF. Increase in p57 Kip2 expression correlates with increased concentration of dexamethasone as described in literature.The cells were incubated at 37Â°C for 24h in media containing different concentrations of (dexamethasone) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA303447 (5 Âµg/ml) was performed overnight at 4Â°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Immunocytochemistry/ Immunofluorescence - Anti-p57 Kip2 antibody [EP2515Y]; ICC/IF image of TA303447 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4Â°C. The secondary antibody (green) was , DyLight? 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43ÂµM.