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Home Antibody All anti-CDKN1B antibodies

Anti-CDKN1B PHOSPHO Antibody EP233(2)Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303446
  • Rabbit monoclonal antibody against p27 (Kip1) (pS10) Phospho (EP233(2)Y ) (phospho-specific)
100ul 325 3-7 Days
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WB(1)
IHC(4)
IF(1)
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Also for CDKN1B (NM_004064)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding Serine 10 of human p27 was used as an immunogen. This antibody detects p27 phosphorylated at Serine 10.
Clone NameEP233(2)Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:10000 - 1:20000; IP: 1:30; IHC-P: 1:200 - 1:1500
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or ICC.

Reference Data

Target NameHomo sapiens cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B)
Alternative NameCDKN4; KIP1; MEN1B; MEN4; P27KIP1
Database LinkNP_004055
FunctionThis gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. [provided by RefSeq].
Related Pathway
Cell cycle
Senescence and Autophagy

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - p27 KIP 1 (phospho S10) antibody [EP233(2)Y]; All lanes : Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] at 1/10000 dilution.Lane 1 : MCF-7 cell lysate (untreated).Lane 2 : MCF-7 cell lysate treated with AP.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 22 kDa.Observed band size : 27 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - p27 KIP 1 (phospho S10) antibody [EP233(2)Y]; Immunohistochemistical detection ofp27 KIP 1 (phospho S10) antibody [EP233(2)Y] on formaldehyde fixedparaffin-embedded monkey cerebellumsections. Antigen retrieval step: Heat mediated.Buffer: Citric acid solution. Permeabilization: None. Blocking step: 1% BSAfor 10 mins. Primary antibody incubated at1/1500 for2 hours@ 21°C inTBS/BSA/azide. Secondary antibody Name: anti rabbit IgG conjugated to biotin (1/200).
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - p27 KIP 1 (phospho S10) antibody [EP233(2)Y]; Immunohistochemistical detection ofp27 KIP 1 (phospho S10) antibody [EP233(2)Y] on formaldehyde-fixed paraffin-embeddedhumantonsil sections.Antigen retrieval step: Heat mediated.Buffer Used: Citric acid. Permeabilization: None. Blocking step: 1% BSAfor 10 mins @21°C. Primary antibodyincubated at1/500 for 2 hours @ 21°Cin TBS/BSA/azide. Notes:A micropolymer HRP kit was used to obtain this intensity of immunostaining as I could not achieve a similar intensity of positivity on human tissues, under the same conditions (compare with results on rat/mouse/marmoset sections, using a std ABCpx detection system). There is one obvious metaphase nucleus in the Germinal centre of the submitted image: it is not positive.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - p27 KIP 1 (phospho S10) antibody [EP233(2)Y]; TA303446 at 1/100 dilution staining p27 KIP 1 in human ovarian carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - p27 KIP 1 (phospho S10) antibody [EP233(2)Y]; TA303446 at 1/100 dilution staining p27 KIP 1 in human tonsils by Immunohistochemistry, Paraffin-embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - p27 KIP 1 (phospho S10) antibody [EP233(2)Y]; ICC/IF image of p27 KIP 1 (phospho S10) staining using TA303446 on Cor1 cells. Cells were fixed for 20 mins using 4% Formalin in PBS. After TBS washes, cells were then permeabilised/blocked simultaneously using antibody diluent before applying primary Ab.

 

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