Homo sapiens cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B)
Synonyms:
CDKN4; KIP1; MEN1B; MEN4; P27KIP1
Immunogen
A phospho specific peptide corresponding to residues surrounding Serine 10 of human p27 was used as an immunogen. This antibody detects p27 phosphorylated at Serine 10.
Buffer
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. [provided by RefSeq].
Related Pathway
Cell cycle
Senescence and Autophagy
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Western blot analysis on MCF-7 cell lysates using anti-Phospho-p27 Kip1 (pS10) RabMAb (TA303446), 1:10,000 dilution. Cells were either (A) untreated (B) treated with AP.
