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Anti-CDK2 PHOSPHO Antibody EP2234Y
Also for CDK2 (NM_001798)
|A synthetic phospho-peptide corresponding to residues surrounding Thr14 of human Cdk2 was used as immunogen. The antibody detects Cdk2 phosphorylated on Threonine 14.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IP: 1:30; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens cyclin-dependent kinase 2 (CDK2), transcript variant 1|
|The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq]. |
Delta-Notch Signaling Pathway
TGF Beta Signaling Pathway
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Western blot - Cdk2 (phospho T14) antibody [EP2234Y]; All lanes : Anti-Cdk2 (phospho T14) antibody [EP2234Y] at 1/20000 dilution.Lane 1 : HeLa cell lysate, untreated.Lane 2 : HeLa cell lysate, treated with hydroxyurea.Lysates/proteins at 10 µg per lane.Secondary.HRP conjugated Goat anti-rabbit at 1/2000 dilution.Predicted band size : 34 kDa.Observed band size : ~34 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cdk2 (phospho T14) antibody [EP2234Y]; Human tonsil tissue stained with ab68265 at 1/100-1/250 dilution.