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Anti-CDH2 Antibody EPR1791-4
Also for CDH2 (NM_001792)
|A synthetic peptide corresponding to residues in the extracellular region of human N-Cadherin was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:5000 - 1:20000; ICC/IF: Use at an assay dependent concentration; FC: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens cadherin 2, type 1, N-cadherin (neuronal) (CDH2)|
|CD325; CDHN; CDw325; NCAD|
|This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq]. |
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Western blot - N Cadherin antibody [EPR1791-4]; Lanes 1 - 2 : Anti-N Cadherin antibody [EPR1791-4] at 1/20000 dilution.Lane 3 : Anti-N Cadherin antibody [EPR1791-4] at 1/5000 dilution.Lane 1 : Huvec cell lysate.Lane 2 : C6 cell lysate.Lane 3 : HeLa cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 100 kDa.Observed band size : 140 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4]; ab76011 stainingN Cadherinin Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with1% BSA + 1% FBS for2 hoursat room temperature; antigen retrieval was by heat mediation in a citrate buffer pH6. Samples were incubated with primary antibody (1/500 in 1% BSA + 1% FBS) for 16 hours at 4°C. An undilutedHRP-conjugatedGoat anti-rabbit IgG polyclonal was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [EPR1791-4]; ab76011 staining N Caherinwild type Mouse embryonic fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.3% and blocked with 3% BSA for 60 minutes at room temperature. Samples were incubated with primary antibody (1/100 in PBS) for 16 hours at 4°C. An Alexa Fluor?488-conjugated Donkey anti-rabbit polyclonal(1/500) was used as the secondary antibody.
Flow Cytometry - N Cadherin antibody [EPR1791-4]; ab76011 (N Cadherin) [EPR1791-4] for Flow Cyt (Mouse)