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Home Antibody All anti-CDC25C antibodies

Anti-CDC25C Antibody E302


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA303434 Rabbit monoclonal antibody against Cdc25C(E302) 100ul $325 In Stock
LC419747 CDC25C HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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Also for CDC25C (NM_001790)
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues near N-terminus of human Cdc25C was used as immunogen. The antibody can also detect splice isoform 2, 4 and 5 of human Cdc25C, based on sequence homology.
Clone NameE302 IsotypeIgG
Species ReactivityRat, Human (Does not react with: Mouse) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:5000; IHC-P: 1:250 - 1:500; ICC/IF: 1:250; FC: Use at an assay dependent dilution; IP: 1:80
Predicted MW Explanation 53 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens cell division cycle 25C (CDC25C), transcript variant 1
Alternative NameCDC25; PPP1R60
Database LinkNP_001781
Entrez Gene 995 Human
Entrez Gene 307511 Rat
FunctionThis gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. [provided by RefSeq].
Related PathwayPhosphataseStem cell - PluripotencyDruggable Genome Cell cycleOocyte meiosisProgesterone-mediated oocyte maturation

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Cdc25C antibody [E302]; Anti-Cdc25C antibody [E302] at 1/5000 dilution + HeLa cell lysate.Predicted band size : 53 kDa.Observed band size : 60 kDa .

IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Cdc25C antibody [E302]; Immunohistochemical analysis of paraffin-embedded human urinary bladder carcinoma TA303434 at 1/250 dilution.

FC Image
Flow Cytometry - Anti-Cdc25C antibody [E302]; Flow cytometry analysis of HeLa cells, staining Cdc25C with TA303434. Cells were fixed with formaldehyde and permeabilized with 90% methanol. Samples were incubated with primary antibody (1/20 in PBS + 10% goat serum) for 1 hour at 23°C. A FITC-conjugated goat anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody.


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