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Home Antibody All anti-CDC25C antibodies

Anti-CDC25C PHOSPHO Antibody E190

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303433
  • Rabbit monoclonal antibody against Phospho-Cdc25C (pS216)(E190) (phospho-specific)
100ul 325 3-7 Days
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WB(2)
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Also for CDC25C (NM_001790)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Ser216 of human Cdc25C was used as immunogen. The antibody only detects Cdc25C phosphorylated on Serine 216.
Clone NameE190 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: 1:1,000; ICC: 1:50; IP: 1:30
Predicted MW Explanation 53 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens cell division cycle 25C (CDC25C), transcript variant 1
Alternative NameCDC25; PPP1R60
Database LinkNP_001781
Entrez Gene 995 Human
FunctionThis gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. [provided by RefSeq].
Related PathwayPhosphataseStem cell - PluripotencyDruggable Genome Cell cycleOocyte meiosisProgesterone-mediated oocyte maturation

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-Cdc25C (phospho S216) antibody [E190]; All lanes : Anti-Cdc25C (phospho S216) antibody [E190] at 1/1000 dilution.Lane 1 : IR treated HeLa cell lysate.Lane 2 : 10 Gy IR treated (0 minutes recovery) HeLa cell lysate.Lane 3 : 10 Gy IR treated (30 minutes recovery) HeLa cell lysate.Lane 4 : 10 Gy IR treated (60 minutes recovery) HeLa cell lysate.Lysates/proteins at 22 ug per lane.developed using the ECL technique.Performed under reducing conditions.Predicted band size : 53 kDa.Exposure time : 5 seconds
WB Image
Western blot - Cdc25C (phospho S216) antibody [E190]; Lane 1 : Anti-Cdc25C (phospho S216) antibody [E190] at 1/1000 dilution (Untreated ).Lane 2 : Anti-Cdc25C (phospho S216) antibody [E190] at 1/1000 dilution (Treated with phosphatase).Lane 1 : As above.Lane 2 : As above.Predicted band size : 53 kDa.Observed band size : 53,60 kDa .

 

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