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Home Antibody All anti-CCNB1 antibodies

Anti-CCNB1 Antibody Y106

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303421
  • Rabbit monoclonal antibody against Cyclin B1 (C-term)(Y106)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC410417) , 20ug
100ul $325 In Stock
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WB(1)
IHC(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in C-terminus of human cyclin B1 was used as immunogen. The antibody does not cross-react with other cyclin family members.
Clone NameY106 IsotypeIgG
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:3000 - 1:20000; IHC-P: Use at an assay dependent concentration; FC: 1:20; ICC: 1:50; IP: 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Protein A purified
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens cyclin B1 (CCNB1)
Alternative NameCCNB
Database LinkNP_114172
FunctionThe protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq].
Related Pathway
Cell cycle
Hedgehog signaling pathway

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WB Image
Western blot - Cyclin B1 antibody [Y106]; All lanes : Anti-Cyclin B1 antibody [Y106] at 1/20000 dilution.Lane 1 : Hela cell lysate.Lane 2 : Jurkat cell lysate.Predicted band size : 58 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Cyclin B1 antibody [Y106]; TA303421 at a 1:100 dilution staining Human cyclin B1 in human skin carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.
FC Image
Flow Cytometry-Cyclin B1 antibody [Y106](TA303421); Overlay histogram showing Jurkat cells stained with TA303421 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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