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Anti-CCNB1 Antibody Y106
Also for CCNB1 (NM_031966)
|A synthetic peptide corresponding to residues in C-terminus of human cyclin B1 was used as immunogen. The antibody does not cross-react with other cyclin family members.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:3000 - 1:20000; IHC-P: Use at an assay dependent concentration; FC: 1:20; ICC: 1:50; IP: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens cyclin B1 (CCNB1)|
|The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq]. |
Hedgehog signaling pathway
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Western blot - Cyclin B1 antibody [Y106]; All lanes : Anti-Cyclin B1 antibody [Y106] at 1/20000 dilution.Lane 1 : Hela cell lysate.Lane 2 : Jurkat cell lysate.Predicted band size : 58 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Cyclin B1 antibody [Y106]; ab32053 at a 1:100 dilution staining Human cyclin B1 in human skin carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.
Flow Cytometry-Cyclin B1 antibody [Y106](ab32053); Overlay histogram showing Jurkat cells stained with ab32053 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.