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Home Antibody All anti-CASP9 antibodies

Anti-CASP9 Antibody E84

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303420
  • Rabbit monoclonal antibody against Pro-Caspase-9(E84)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC409796) , 20ug
100ul 325 In Stock
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WB(1)
IHC(1)
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Also for CASP9 (NM_032996)
cDNA Clone shRNA/siRNA Protein/Lysate Antibody Assay

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near N-terminus of human Caspase-9 was used as immunogen. This antibody only recognises the Pro form of Caspase-9 (46kDa).
Clone NameE84 IsotypeProtein A purified
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:2000; FC: 1:50; IHC-P: 1:25
BufferDoes not react with Mouse, Rat. Is unsuitable for ICC or IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for ICC or IP.

Reference Data

Target NameHomo sapiens caspase 9, apoptosis-related cysteine peptidase (CASP9), transcript variant 3
Alternative NameAPAF-3; APAF3; ICE-LAP6; MCH6; PPP1R56
Database LinkNP_127463
FunctionThis gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms. [provided by RefSeq].
Related Pathway
Apoptosis
EGFR1 Signaling Pathway
MAPK signaling pathway

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WB Image
Western blot - pro Caspase 9 antibody [E84]; Anti-pro Caspase 9 antibody [E84] at 1/2000 dilution + Jurkat cell lysate at 10 µg.Predicted band size : 46 kDa.Observed band size : 46 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pro Caspase 9 antibody [E84]; Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using anti-Pro-Caspase-9 RabMAb.

 

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